刺猬（Hh）由N-末端和C-末端结构域组成,在自催化的蛋白水解裂解反应中解离.N-末端产物HHNP在自我分裂过程中被胆固醇修饰,窝藏所有已知的HH信号传导活性.当在没有C端结构域（因此缺乏胆固醇修饰）的情况下合成时,N端结构域被异常靶向并选择性地释放到视网膜中.Hedgehog信号通路与肿瘤发生相关,在多种癌症中异常激活.Hh配体结合并抑制跨膜受体补丁（PTCH）,PTCH抑制平滑（SMO）,一种积极调节Hh通路的七跨膜螺旋蛋白.
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